Methods for determining levels of 1,25 dihydroxy vitamin d2 and d3
OwnerRobert L. Taylor, Ravinder J. Singh +3inventors
InventorsRobert L. Taylor, Ravinder J. Singh, Kendall W. Cradic, James L. Bruton, JR., Brian C. Netzel
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Abstract
Methods for determining the amount of DHVD2 and/or DHVD3 in a sample are provided. The methods can employ LC-MS/MS techniques coupled with sample affinity purification and derivatization steps. Methods for diagnosing vitamin D deficiencies are also provided.
Claims25
A method for determining an amount of DHVD2 in a sample, wherein the method comprises:
- a) subjecting the sample to an affinity purification step comprising contacting the sample with an antibody specific for DHVD2 to form an affinity purified sample;
- b) derivatizing the DHVD2 from the affinity purified sample to form a derivatized sample;
- and
- c) subjecting the derivatized sample to a MS technique to determine the amount of DHVD2.
A method for determining whether or not a mammal has a vitamin D deficiency, the method comprising determining the amount of DHVD2 and DHVD3 in a sample from the mammal.
A method for determining whether or not a mammal has hypervitaminosis D, the method comprising determining the amount of DHVD2 and DHVD3 in a sample from the mammal.
A method for monitoring vitamin D replacement therapy in a mammal, the method comprising determining the amount of DHVD2 in a sample from the mammal.
Dependencies are inferred from the claim text.