Mass Spectrometry-Based Protein Identification Method
AssigneeRudjer Bosckovic Institute
InventorsMario Cindric, Sandra Kraljevic Pavelic, Anita Horvatic, Ivana Dodig
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Abstract
A method of detection of amino acid sequences and/or identification of proteins and peptides is based on derivatization of peptides or proteins using compounds comprising two or more sulfonyl groups, and subsequent analysis of the derivatized analytes using a mass spectrometer in its negative mode of operation.
Claims16
A method of detection of an amino acid sequence for identification of peptides and/or proteins having an N-terminus, comprising the following steps:
- derivatization of a peptide and/or protein at its N-terminus using a derivatization compound comprising two or more sulfonyl groups and a reactive group binding to an amino group at the N-terminus of the peptide and/or protein to form one or more derivatized analytes;
- mass spectrometer analysis of the one or more derivatized analytes by acquiring spectra of derivatized negative ions in a negative mode of operation of a mass spectrometer;
- determining an amino acid sequence of one or more derivatized analytes by interpretation of fragmentation sequence data obtained from the mass spectrometer analysis.
(canceled)
A method of detection of an amino acid sequence for identification of pep-tides and/or proteins having an N-terminus, comprising the following steps:
- derivatization of a peptide and/or protein at its N-terminus using 4-formylbenzene-1,3-disulfonic acid as a derivatization compound to bind to an amino group at the N-terminus of the peptide and/or protein to form one or more derivatized analytes;
- mass spectrometer analysis of the one or more derivatized analytes by acquiring spectra of derivatized negative ions in a negative mode of operation of a mass spectrometer;
- determining an amino acid sequence of one or more derivatized analytes by interpretation of fragmentation sequence data obtained from the mass spectrometer analysis.
Dependencies are inferred from the claim text.