Muscle-specific expression vectors
OwnerDavid Souza, Donna Armentanoinventors
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Abstract
The invention is directed to novel combinations of muscle-specific enhancers and promoter elements useful for achieving persistent expression in the muscle or myocyctes. The muscle-specific promoter elements are derived from a muscle creatine kinase promoter, a troponin I promoter, a skeletal alpha-actin promoter, or a desmin promoter. The muscle-specific enhancer elements are derived from either troponin I internal regulatory elements, muscle creatine kinase enhancers, or desmin enhancers.
Claims46
A recombinant muscle-specific regulatory element for gene expression, comprising:
- (a) a promoter element selected from the group consisting of the mammalian muscle creatine kinase (MCK) promoter, human muscle creatine kinase promoter, and mammalian desmin (DES) promoter;
- and
- (b) at least one two enhancer elements selected from the group consisting of mammalian muscle creatine kinase (MCK) enhancer, mammalian DES enhancer, and vertebrate troponin I IRE (FIRE) enhancer,
- wherein the promoter and enhancer elements are operably linked.
A recombinant transgene useful for expression of a coding sequence, comprising a strong constitutive promoter and one or more muscle-specific enhancer elements, wherein the strong constitutive promoter is selected from the group consisting of mammalian desmin (DES) promoter, mammalian muscle creatine kinase (MCK) promoter, mammalian troponin I (TNNI12) promoter, and mammalian skeletal alpha-action (ASKA) promoter and the muscle-specific enhancer is selected from the group consisting of mammalian troponin I internal regulatory elements (FIRE), mammalian muscle creatine kinase (MCK) enhancers, and mammalian desmin (DES) enhancers.
Dependencies are inferred from the claim text.