Novel substitution mutant receptors and their use in an ecdysone receptor-based inducible gene expression system
AssigneeIntrexon Corporation
InventorsSubba Reddy Palli, Marianna Z. Kapitskaya
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Abstract
This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.
Claims36
A gene expression modulation system comprising
- a) a polynucleotide that encodes a first polypeptide comprising
- a Group B nuclear receptor ligand binding domain comprising a substitution mutation, and
- b) a polynucleotide that encodes a second polypeptide comprising a nuclear receptor ligand binding domain that dimerizes with said Group B nuclear receptor ligand binding domain comprising a substitution mutation.
A gene expression cassette comprising a polynucleotide that encodes a polypeptide selected from the group consisting of
- a) a polypeptide comprising a transactivation domain, a DNA-binding domain, and a Group B nuclear receptor ligand binding domain comprising a substitution mutation,
- b) a polypeptide comprising a DNA binding domain and a Group B nuclear receptor ligand binding domain comprising a substitution mutation, and
- c) a polypeptide comprising a transactivation domain and a Group B nuclear receptor ligand binding domain comprising a substitution mutation.
An isolated polynucleotide encoding a Group B nuclear receptor ligand binding domain comprising a substitution mutation, wherein the isolated polynucleotide comprises a codon mutation that results in a substitution of an amino acid residue at a position equivalent to or analogous to a) 401 or 429 of SEQ ID NO:
- 1, b) 401 and 429 of SEQ ID NO:
- 1, c) 337, 344, 355, 385, 431, 442, 462, 470, 472, 473, 495, 500, 511, 516, or 528 of SEQ ID NO:
- 2, d) 321, 322, and 323 of SEQ ID NO:
- 2, e) 450, 451, and 452 of SEQ ID NO:
- 2, f) 455, 456, 457, and 458 of SEQ ID NO:
- 2, g) 470, 472, and 473 of SEQ ID NO:
- 2, h) 475, 476, 477, 478, and 479 of SEQ ID NO:
- 2, and i) 481, 482, and 483 of SEQ ID NO:
- 2.
(canceled)
(canceled)
Dependencies are inferred from the claim text.