Method and apparatus for substantially isolating plant tissues
AssigneeMonsanto Technology LLC
InventorsWhitney R. Adams, JR., Brandon Davis, Lubomyr Kucher, Brian Martinell, Jyoti Rout, Brenda Lowe
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Abstract
The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
Claims12
1-11. (canceled)
A method for obtaining embryos suitable for tissue culture and/or genetic transformation, comprising at least partially excising an embryo from a plant seed in a liquid medium consisting essentially of water and/or an osmotic agent with an osmolality of about 0 mOsm/kg to about 600 mOsm/kg, wherein the embryo remains viable for tissue culture and/or genetic transformation following excising the embryo from the plant seed, comprising preparing the liquid medium in a media preparation system comprising:
- (a) an inlet for water;
- (b) an inlet for the osmotic agent;
- and
- (c) a chamber for mixing the water and osmotic agent to produce the liquid medium, wherein the inlet for water and the inlet for osmotic agent are coupled to the chamber for mixing to allow delivery of the water and osmotic agent to the chamber for mixing.
22-73. (canceled)
Dependencies are inferred from the claim text.