Biomaterial and preparation method thereof
AssigneeBody Organ Biomedical Corp.
InventorsHorng-Ji LAI, Chien-Cheng LIN, Shang-Ming LIN
Life of this patent
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Abstract
A method for preparing a biomaterial comprising the steps of acellularizing the fish scale to remove cell components, decalcifying the fish scale; and cleaning the fish scale, wherein in the step of acellularizing, it further including stirring constantly the fish scale in a solution comprised of an octylphenoxypolyethoxyethanol, a tris-buffered salt and a protease inhibition; and rinsing in a Hanks' buffered saline and the fish scale keeps the naturally 3-dimension microstructure after the step of acellularizing the fish scale.
Claims19
A method for preparing a biomaterial from a fish scale, comprising the steps of:
- acellularizing the fish scale to remove a cell component, wherein the fish scale keeps the naturally 3-dimension microstructure after the step of acellularizing the fish scale;
- cleaning the fish scale;
- and
- decalcifying the fish scale, wherein in the step of acellularizing, it further including stirring constantly the fish scale in a solution with an octylphenoxypolyethoxyethanol;
- and rinsing in a Hanks' buffered saline.
A method for corneal regeneration, comprising steps of:
- acellularizing the fish scale to remove a cell component, wherein the fish scale keeps the naturally 3-dimension microstructure after the step of acellularizing the fish scale;
- cleaning the fish scale;
- decalcifying the fish scale to serve as a scaffold;
- harvesting a corneal;
- seeding the corneal onto the scaffold in a plate;
- and
- culturing a cell of the corneal, wherein in the step of acellularizing, it further includes stirring constantly the fish scale in a solution with an octylphenoxypolyethoxyethanol;
- and rinsing in a Hanks' buffered saline.
The method according to claim 11, wherein in the step of cleaning the fish scale, it further includes running a Limulus Amebocyte Lysate (LAL) test and repeating the step of cleaning the fish scale and running the LAL test until a value of LAL of the fish scale is below 200 Eu/mg.
The method according to claim 12, further comprising a step of dehydrating the fish scale after cleaning the fish scale and the fish scale is performed until the fish scale containing less than about 25% of water.
Dependencies are inferred from the claim text.