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AssigneeWheaton Industries
InventorsJohn R. Wilson, Daniel P. Welch, Alison Robeck, Douglas A. Page
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A versatile compartmentalized cell culture device, with a selectively permeable membrane separating the compartments, provides many attributes relative to traditional devices. It can be configured for high-density cell culture, co-culture, and sample dialysis while rolling or standing still. It can also be configured for continuous movement of liquid between compartments. The wide combination of attributes not found in other membrane based cell culture and bioprocessing devices includes more cell capacity, more cell secreted product capacity, higher cell and product density, increased medium capacity, minimized use of exogenous growth factors, compatibility with standard cell culture equipment and protocols, increased scale up efficiency, capacity to function when rolling or standing still, capacity for perfusion without the need for pumps, and more efficient sample dialysis.
A method of culturing cells in a compartmentalized roller bottle including a basal medium compartment and a cell culture compartment residing within a roller bottle housing, an access port to said cell culture compartment, and an access port to said basal medium compartment, said roller bottle housing having a first end, a second end, and a sidewall connecting said first end to said second end, said cell culture compartment included in the volume of space within said roller bottle housing that is not occupied by said basal medium compartment, said cell culture compartment being bounded by at least a portion of said sidewall of said roller bottle housing, at least a portion of said basal medium compartment being comprised of a non-microporous semi-permeable membrane, the method comprising
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