Expression vector and methods of producing high levels of proteins
AssigneeCadila Healthcare Limited
InventorsArun K. Singh, Ashish Goel, Sanjeev K. Mendiratta
Life of this patent
Estimating the term…
Abstract
A process for high expression of protein of interest using an expression vector. The process comprises at least the following regulatory elements: a) a CMV promoter, or its functional variants, b) an intron, c) TPL or its functional variants, d) VA genes or functional variants, and e) a bovine growth hormone polyadenylation sequence or functional variants.
Claims24
A process for high expression of protein of interest using an expression vector which comprises at least the following regulatory elements:
- a) a CMV promoter, or its functional variants,
- b) an intron,
- c) TPL or its functional variants,
- d) VA genes or functional variants, and
- e) a bovine growth hormone polyadenylation sequence or functional variants.
Use of the vectors as claimed in any of the preceding claims for obtaining high expression of proteins of interest.
Recombinant erythropoietin prepared according to the process as claimed in any of the preceding claims.
Recombinant fusion protein TNFR-IgGFc prepared according to the process as claimed in any of the preceding claims.
Monoclonal antibodies selected from rituximab, trastuzumab, bevacuzumab prepared according to the process as claimed in any of the preceding claims.
A protein(s) encoded by a suitable gene(s) prepared according to the process as claimed in any of the preceding claims.
Dependencies are inferred from the claim text.