Novel Synthetic Expression Vehicle
OwnerLaxmi Srinivas Rao, Shrikant Mishra +3inventors
InventorsLaxmi Srinivas Rao, Shrikant Mishra, Milind Niphadkar, Priti Thakur, Genevieve Nazareth
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Abstract
An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E. coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between −55 to −10 and ribosome binding site from the transcription initiation site.
Claims12
1) An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E. coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between −55 to −10 and ribosome binding site from the transcription initiation site.
4) An expression vector comprising a synthetic hybrid promoter as shown in Seq ID No. 1.
7) A prokaryotic host cell harboring the expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1.
9) A prokaryotic host cell harboring the expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 with an ATCC accession no. PTA-9371.
11) A prokaryotic host cell harboring a high plasmid copy number expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 with an ATCC accession no. PTA-9371.
12) The expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 as substantially described herein with respect to the foregoing examples 1 to 13 and drawings 1 to 7.
Dependencies are inferred from the claim text.