US 2011/0212506 A1 · NITRILE HYDRATASE VARIANT

Abstract

A nitrile hydratase variant of the present invention comprises substitution of at least one amino acid with another amino acid to improve two or more properties of nitrile hydratase by substitution of one amino acid.

Claims17

Dependent claims

A nitrile hydratase variant comprising substitution of at least one amino acid with another amino acid to improve two or more properties of nitrile hydratase by substitution of one or more and three or less amino acids.

A gene encoding a nitrile hydratase variant having a gene encoding the α-subunit defined in SEQ ID No:

3 in the Sequence Listing and a gene encoding the β-subunit defined in SEQ ID No:
4 in the Sequence Listing, comprising substitution of at least one base selected from substitution sites of the base consisting of the following (a) to (l):
(a) 274th to 276th of the base sequence of SEQ ID No:
3;
(b) 280th to 282nd of the base sequence of SEQ ID No:
3;
(c) 589th to 591st of the base sequence of SEQ ID No:
3;
(d) 10th to 12th of the base sequence of SEQ ID No:
4;
(e) 69th to 71st of the base sequence of SEQ ID No:
4;
(f) 235th to 237th of the base sequence of SEQ ID No:
4;
(g) 286th to 288th of the base sequence of SEQ ID No:
4;
(h) 319th to 321st of the base sequence of SEQ ID No:
4;
(i) 676th to 678th of the base sequence of SEQ ID No:
4;
(j) 328th to 330th of the base sequence of SEQ ID No:
4 and 691st to 693rd of the base sequence of SEQ ID No:
4;
(k) 616th to 618th of the base sequence of SEQ ID No:
4, and 688th to 690th of the base sequence of SEQ ID No:
4;
and
(l) 37th to 39th of the base sequence of SEQ ID No:
3, 79th to 81st of the base sequence of SEQ ID No:
3, and 328th to 330th of the base sequence of SEQ ID No:
4.

28 more elements

Dependencies are inferred from the claim text.

Abstract

Claims17

Dependent claims

A gene encoding a nitrile hydratase variant having a gene encoding the α-subunit defined in SEQ ID No:

3 in the Sequence Listing and a gene encoding the β-subunit defined in SEQ ID No:
4 in the Sequence Listing, comprising substitution of at least one base selected from substitution sites of the base consisting of the following (a) to (l):
(a) 274th to 276th of the base sequence of SEQ ID No:
3;
(b) 280th to 282nd of the base sequence of SEQ ID No:
3;
(c) 589th to 591st of the base sequence of SEQ ID No:
3;
(d) 10th to 12th of the base sequence of SEQ ID No:
4;
(e) 69th to 71st of the base sequence of SEQ ID No:
4;
(f) 235th to 237th of the base sequence of SEQ ID No:
4;
(g) 286th to 288th of the base sequence of SEQ ID No:
4;
(h) 319th to 321st of the base sequence of SEQ ID No:
4;
(i) 676th to 678th of the base sequence of SEQ ID No:
4;
(j) 328th to 330th of the base sequence of SEQ ID No:
4 and 691st to 693rd of the base sequence of SEQ ID No:
4;
(k) 616th to 618th of the base sequence of SEQ ID No:
4, and 688th to 690th of the base sequence of SEQ ID No:
4;
and
(l) 37th to 39th of the base sequence of SEQ ID No:
3, 79th to 81st of the base sequence of SEQ ID No:
3, and 328th to 330th of the base sequence of SEQ ID No:
4.

28 more elements

Dependencies are inferred from the claim text.

Nitrile hydratase variant

Life of this patent

Abstract

Claims17

Nitrile hydratase variant

Life of this patent

Abstract

Claims17