BGL3 Beta-Glucosidase and Nucleic Acids Encoding the Same
AssigneeDanisco US Inc.
InventorsNigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Abstract
The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.
Claims7
-17. (canceled)
A substantially purified BGL3 polypeptide with the biological activity of a β-glucosidase, comprising a sequence selected from the group consisting of:
- (a) an amino acid sequence having at least 85% sequence identity to the amino acid sequence presented in FIG. 2 (SEQ ID NO:2);
- (b) an amino acid sequence having at least 90% sequence identity to the amino acid sequence presented in FIG. 2 (SEQ ID NO:2);
- (c) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented in FIG. 2;
- (d) an amino acid sequence presented in FIG. 2;
- (e) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2;
- (f) an amino acid sequence presented as SEQ ID NO:2;
- (g) a substantially purified biologically active fragment of the amino acid sequence presented as SEQ ID NO:2.
-20. (canceled)
A purified enzyme having β-glucosidase activity prepared by the method of claim 19.
-24. (canceled)
A detergent composition, said composition comprising a polypeptide selected from the group consisting of:
- (a) an amino acid sequence having at least 85% sequence identity to the amino acid sequence presented in FIG. 2 (SEQ ID NO:2);
- (b) an amino acid sequence having at least 90% sequence identity to the amino acid sequence presented in FIG. 2 (SEQ ID NO:2);
- (c) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented in FIG. 2;
- (d) an amino acid sequence presented in FIG. 2;
- (e) an amino acid sequence having at least 95% sequence identity to the amino acid sequence presented as SEQ ID NO:2;
- (f) an amino acid sequence presented as SEQ ID NO:2;
- (g) a substantially purified biologically active fragment of the amino acid sequence presented as SEQ ID NO:2.
-28. (canceled)
Dependencies are inferred from the claim text.