Recombinant expression of carboxylesterases
AssigneeTongji University
InventorZhongbin Liu
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Abstract
The present application provides a method of producing a carboxylesterase or its variant in eukaryotic cells. The present application also provides an expression vector for high level carboxylesterase expression, a eukaryotic cell comprising the expression vector, and uses thereof. The present application also provides a composition comprising a carboxylesterase or its variant produced by a method described in the present application and uses of the composition.
Claims22
A method for producing a protein, comprising:
- culturing a eukaryotic cell engineered to express a gene encoding a thermostable carboxylesterase from a microbe or its variant under conditions suitable for expression of the thermostable carboxylesterase or its variant.
(canceled)
18-31. (canceled)
A recombinant eukaryotic cell comprising an expression vector comprising a gene encoding a microbial thermostable carboxylesterase or its variant;
- and a regulatory sequence capable of promoting expression of the microbial thermostable carboxylesterase or its variant in a eukaryotic cell, wherein the regulatory sequence is operably linked to the gene.
35-37. (canceled)
Dependencies are inferred from the claim text.