Method for designing heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase and heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase
AssigneeSony Corporation
InventorsDaisuke Yamaguchi, Seiji Yamada, Yoshio Goto, Yuichi Tokita
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Abstract
A method for designing a heat-resistant mutant enzyme, the method including the step of reducing a distance between the α4 helix and the α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase.
Claims7
A method for designing a heat-resistant mutant enzyme, the method comprising:
- reducing a distance between α4 helix and α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase.
A heat-resistant mutant tyrosine-dependent short-chain dehydrogenase/reductase,
- wherein a distance between the α4 helix and the α6 helix in a protein three-dimensional structure is smaller than that of a wild type tyrosine-dependent short-chain dehydrogenase/reductase.
A heat-resistant mutant gluconic acid dehydrogenase comprising
- an amino acid sequence indicated by Seq ID No. 1, in which one or several amino acids are subjected to deletion, replacement, addition, or insertion,
- wherein a distance between the α4 helix and the α6 helix in a protein three-dimensional structure is smaller than that of a wild type gluconic acid dehydrogenase having the amino acid sequence indicated by Seq ID No. 1.
An electrochemical apparatus by using an enzyme,
- wherein the enzyme is a heat-resistant mutant tyrosine-dependent short-chain dehydrogenase/reductase having a distance between α4 helix and α6 helix in the protein structure smaller than that of a wild type tyrosine-dependent short-chain dehydrogenase/reductase.
Dependencies are inferred from the claim text.