3-dimensional multi-layered hydrogels and methods of making the same
AssigneeThe Brigham and Women's Hospital, Inc.
InventorSeung-Schik Yoo
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Abstract
Embodiments of the invention provide three dimensional multi-layered hydrogel constructs with embedded channels, living cells and bioactive agents, and methods for making three dimensional multi-layered hydrogel constructs. The constructs can have bioactive agents to support the living cells. The multi-layered constructs can have channels for perfusion purposes and layers of different hydrogel materials.
Claims26
A method of making a three dimensional multi-layered hydrogel construct, the method comprising the steps of:
- a. applying a first nebulized layer of cross-linking material on a substrate;
- b. depositing at least one layer of hydrogel precursor on top of the first nebulized layer of cross-linking material, wherein the hydrogel precursor cross-links upon contact with the nebulized layer of cross-linking material to form a partially cross-linked gel;
- c. applying a second nebulized layer of cross-linking material on top of the partially cross-linked gel of step (b), thereby promoting completing cross-linking of the layer of hydrogel of step (b);
- and
- d. repeating alternating step b followed by step (c).
A three dimensional multi-layered hydrogel construct comprising at least 10 layers of hydrogel material, at least one type of cells, wherein the cells are deposited on different layers of hydrogel material, and at least one type of hydrogel material.
Dependencies are inferred from the claim text.