Culture medium for eukaryotic cells
AssigneeCampina Nederland Holding B.V.
InventorsMireille Maria Gadellaa, Edward Allen Hunter
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Abstract
A proteolysate of a seed material derived from a plant species of the Asteraceae family, such as sunflower, has improved properties as a constituent of a culture medium for culturing eukaryotic, in particular animal cells. The seed material is defatted and is hydrolysed to a degree of 10-50% and subsequently separated from insolubles. The cells are particularly cultured for producing desired protein products.
Claims19
-15. (canceled)
A culture medium for culturing eukaryotic cells comprising a hydrolysate of a protein-containing seed material derived from a plant species of the genus Helianthus, Carthamus or Vernonia.
A culture medium for culturing eukaryotic cells comprising:
- (a) 2-80 wt. %, on a dry weight basis, of a hydrolysate of a defatted seed material derived from a plant species of the genus Helianthus, Carthamus or Vernonia;
- and
- (b) at least one cytokine, growth factor, or both.
A liquid culture medium for culturing eukaryotic cells comprising 0.05-1.0 wt/vol % of a hydrolysate of a defatted seed material derived from a plant species of the genus Helianthus, Carthamus or Vernonia.
A method of culturing eukaryotic cells in vitro, comprising growing the eukaryotic cells in a culture medium comprising a hydrolysate of a seed material derived from a plant species of the genus Helianthus, Carthamus or Vernonia.
A method for producing a protein product, the method comprising:
- (a) culturing eukaryotic cells producing the protein product in a culture medium comprising a hydrolysate of a seed material derived from a plant species of the genus Helianthus, Carthamus or Vernonia;
- and
- (b) recovering the protein product from the culture medium.
A kit for eukaryotic cell culture comprising:
- (a) a composition comprising a hydrolysate of protein-containing seed material of a plant species of the genus Helianthus, Carthamus or Vernonia, and
- (b) a composition comprising cytokines, growth factors, vitamins, minerals, amino acids, buffering salts, trace elements, nucleosides, nucleotides, phytohormones, sugars, antibiotics, or combinations thereof,
- wherein the compositions are to be mixed prior to use.
Dependencies are inferred from the claim text.