Immortalized avian cell lines and use thereof
AssigneeTransgene S.A.
InventorsJean-Marc BALLOUL, Marina KAPFER, Thierry MENGUY
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Abstract
The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen (W—COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxyiridae. The invention further relates to the use of said immortalized avian cell lines and related methods for producing biologics, including viruses and proteins.
Claims24
A method for producing virus, comprising the steps of:
- (a) providing an immortalized avian cell line;
- (b) infecting said immortalized avian cell line with a virus to be produced;
- and
- (c) cultivating the said infected avian cell line under conditions which are enabling virus amplification;
- wherein said immortalized avian cell line is selected in the group consisting of immortalized avian cell line deposited at the European Collection of Cell Cultures (ECACC) under accession number 08060502, an immortalized avian cell line deposited at the ECACC under accession number 08060501 and derivatives thereof.
An immortalized avian cell line characterized in being able of amplifying at least one Flaviviridae strain while being unable of amplifying Vaccinia virus strain Copenhagen (W—COP) nor Modified Vaccinia virus Ankara (MVA).
An immortalized avian cell line characterized in being able of amplifying (i) at least one Flaviviridae strain and (ii) at least one Poxyiridae strain.
Use of the immortalized avian cell line deposited at the European Collection of Cell Cultures (ECACC) under accession number 08060502 and derivatives thereof, for the production of viruses and proteins.
Use of the immortalized avian cell line deposited at the European Collection of Cell Cultures (ECACC) under accession number 08060501 and derivatives thereof, for the production of viruses and proteins.
A method for producing a virus comprising the steps of:
- a) infecting an immortalized avian cell line deposited at the European Collection of Cell Cultures (ECACC) under accession number 08060502, with a virus;
- and/or
- b) cultivating the infected avian cell line under conditions which are enabling virus amplification.
A method for producing a virus comprising the steps of:
- a) infecting an immortalized avian cell line deposited at the European Collection of Cell Cultures (ECACC) under accession number 08060501, with a virus;
- and/or
- b) cultivating the infected avian cell line under conditions which are enabling virus amplification.
A method for producing a protein comprising the steps of:
- a) contacting an immortalized avian cell line deposited at the European
- Collection of Cell Cultures (ECACC) under accession number 08060502, with a recombinant vector comprising a nucleotide sequence coding the protein;
- and/or
- b) cultivating the avian cell line under conditions which are enabling the protein to be produced.
A method for producing a protein comprising the steps of:
- a) contacting an immortalized avian cell line deposited at the European Collection of Cell Cultures (ECACC) under accession number 08060501, with a recombinant vector comprising a nucleotide sequence coding the protein;
- and/or
- b) cultivating the avian cell line under conditions which are enabling the protein to be produced.
Dependencies are inferred from the claim text.