Methods and Kits for the Diagnosis of RyR1-Related Diseases
AssigneeThe Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
InventorsSaiid Bina, Rolf Bunger
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Abstract
The present invention encompasses methods for diagnosis of calcium channel related diseases (e.g., RyR1-related diseases) comprising contacting lymphocytes isolated from a subject with a calcium channel agonist (e.g., an RyR1 agonist), measuring the adenosine and inosine produced by the lymphocytes; and comparing the measured levels in the sample to the adenosine and inosine levels in a normal control, whereby an increase in the adenosine and inosine levels relative to the control is indicative of a calcium channel related disease (e.g., RyR1-related disease). The invention also encompasses kits for diagnosis of calcium channel-related diseases.
Claims20
A method for detecting a calcium channel related disease in a subject comprising:
- (a) contacting lymphocytes isolated from the subject with a calcium channel agonist;
- (b) measuring the adenosine and inosine produced by the lymphocytes;
- and
- (c) comparing the measured levels in the sample to the adenosine and inosine levels in a normal control, wherein an increase in the adenosine and inosine levels relative to the control is indicative of a calcium channel related disease.
A kit for detecting a calcium channel related disease in a subject comprising (1) a calcium channel agonist, (2) a normal control comprising a sample of solution containing levels of adenosine and inosine equivalent to levels produced by lymphocytes from a normal individual and (3) a positive control comprising levels of adenosine and inosine equivalent to levels produced by lymphocytes from a subject with a calcium channel related disease.
Dependencies are inferred from the claim text.