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US 2011/0212480 A1Published application
Use of primary human cardiomyocytes
OwnerKyle Louis Kolaja, Preeti Dhawan +1inventors
InventorsKyle Louis Kolaja, Preeti Dhawan, Hirdesh Uppal
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Abstract
The present invention provides for the use of primary human cardiomyocytes for the testing of agents that have cardiotoxic effects and other modulatory effects on the heart.
Claims10
A method of screening an agent for a modulatory effect on primary human cardiomyocytes comprising:
- a) culturing said primary human cardiomyocytes,
- b) plating said primary human cardiomyocytes on multi-well plates selected from the group consisting of 6-well plates, 24-well plates, 48-well plates, 96-well plates, and 384-well plates.
- c) contacting said primary human cardiomyoctyes with the agent;
- and
- d) examining said primary human cardiomyocytes for the modulatory effect resulting from said agent.
2 more elements
A method of screening an agent for a modulatory effect on primary human cardiomyocytes comprising:
- a) culturing primary human cardiomyocytes for at least three passages (P=3) in growth medium comprising of Medium 199 or DMEM, 2-10% bovine or fetal calf serum, on plates coated with collagen I;
- b) plating said primary human cardiomyocytes on multi-well plates selected from the group consisting of 6-well plates, 24-well plates, 48-well plates, 96-well plates, and 384-well plates;
- c) contacting said primary human cardiomyoctyes with the agent;
- and
- d) examining said primary human cardiomyocytes for the modulatory effect resulting from said agent.
2 more elements
Dependencies are inferred from the claim text.