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US 2011/0212453 A1Published application
Assay card for sample acquisition, treatment and reaction
OwnerAbhishek K. Agarwal, Sujit R. Jangam +2inventors
InventorsAbhishek K. Agarwal, Sujit R. Jangam, Kunal Sur, David M. Kelso
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Abstract
The present disclosure relates to devices and systems and methods for their use for detecting an analyte. In particular, the present disclosure provides a disposable assay card in which reaction reagents are stored within the card to facilitate point-of-care application.
Claims32
An assay device, comprising:
- a planar reaction card comprising a sample port for receiving a sample into a reaction chamber;
- a reagent input port on the card for receiving a reagent, the reagent input port in fluid communication with the reaction chamber,
- at least one reagent reservoir in fluid communication with the reagent input port and attached to the card;
- and
- an optical window positioned on the card for monitoring a reaction in said reaction chamber.
2 more elements
An assay card, comprising:
- a sample introduction module movably engaged with an opening on the assay card, said sample introduction module forming a liquid-tight seal with the opening when engaged therein;
- the sample introduction module movable from a first position to at east one subsequent position in the assay card;
- and
- one or more reagent reservoirs positioned on the assay card for release of a component when the sample introduction module is in the first position or the at least one subsequent position.
1 more element
A kit, comprising:
- (a) a planar reaction card comprising;
- a sample port for receiving a sample into a reaction chamber;
- a reagent input port on the card for receiving a reagent, the reagent input port in fluid communication with the reaction chamber,
- an optical window positioned on the card for monitoring a reaction between the sample and the reagent in said reaction chamber;
- (b) one or more reagent reservoirs configured to be affixed to the reaction card for release of a liquid component contained in a storage chamber on each of the one or more reagent reservoirs into the reagent input port of the reaction card;
- (c) a sample introduction module configured for insertion into the sample port on the reaction card.
3 more elements
A system, comprising:
- (a) an assay card, comprising
- i) a port for receiving a sample introduction module;
- ii) at least one regent reservoir comprising reagent in liquid form, the reagent reservoir positioned on the assay card to release its liquid reagent into a reagent input port on the assay card;
- iii) a reaction chamber in fluid communication with the reagent input port, the reaction chamber configured to receive the sample introduction module upon insertion of the sample introduction module into the port;
- and
- iii) an optical window positioned on at least a portion of the reaction chamber for monitoring a reaction in said reaction chamber, and
- (b) an analyzer adapted for receiving the assay card, the analyzer comprising a thermal cycler, an electromechanical component to apply a force to the at least one reagent reservoir to effect release of the liquid reagent, and an optical system.
4 more elements
A method for detecting an analyte in a sample, comprising:
- providing an assay card, comprising
- a) a sample port for receiving a sample into a reaction chamber;
- b) a reagent input port on the card for receiving a reagent, the reagent input port in fluid communication with the reaction chamber,
- c) an optical window positioned on the card for monitoring a reaction between the sample and the reagent in said reaction chamber;
- d) one or more reagent reservoirs configured to be affixed to the reaction card for release of a liquid reagent contained in a storage chamber on each of the one or more reagent reservoirs into the reagent input port of the reaction card;
- and
- e) a sample introduction module configured for insertion into the sample port on the reaction card;
- placing a sample on the assay card;
- conducting a reaction in the reaction chamber between the sample and the reagent;
- and
- monitoring the reaction optically via the optical window to detect the presence or absence of an analyte in the sample.
8 more elements
Dependencies are inferred from the claim text.