Method for preparative production of long nucleic acids by pcr
AssigneeRiNA-NETZWRK RNA-Technologien GmbH
InventorsHelmut Merk, Vlker Erdmann, Wolfgang Stiege
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.
Claims12
A method for preparative production of long nucleic acids by means of PCR and involving the following hybridization steps:
- a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer,
- b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence,
- wherein a nucleic acid sequence enlarged by the extension sequences and amplified on the 3′ and 5′ ends of the nucleic acid base sequence is formed from the nucleic acid base sequence.
A nucleic acid for cell-free protein biosynthesis systems which contains a base sequence coding for a protein and a ribosomal binding sequence as well as an option one or several sequences of the group comprising “promoter sequence, transcription terminator sequence, expression enhancer sequence, stabilization sequence and affinity tag sequence”.
Dependencies are inferred from the claim text.