Swi5 gene as a diagnostic target for the identification of fungal and yeast species
AssigneeNational University of Ireland, Galway
InventorsThomas Gerard Barry, Majella Maher, Terry James Smith, Marcin Jankiewicz, Louise O'Connor, Nina Tuite, Sinead Lahiff
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Abstract
The invention relates to the SWI5 gene, the corresponding RNA, specific probes, primers and oligonucleotides related thereto and their use in diagnostic assays to detect and/or discriminate between fungal and yeast species.
Claims21
A diagnostic kit for a fungal or yeast species comprising an oligonucleotide probe capable of binding to at least a portion of the SWI5 gene or its corresponding mRNA.
A nucleic acid molecule selected from the group consisting of:
- SEQ ID NO 1 through SEQ ID NO 95 and sequences substantially homologous or substantially complementary thereto or to a portion thereof and having a function in diagnostics based on the SWI5 gene.
A method of detecting a target organism in a test sample comprising the steps of:
- (i) Mixing the test sample with at least one oligonucleotide probe capable of binding to at least a portion of the SWI5 gene or its corresponding mRNA under appropriate conditions;
- (ii) hybridizing under a high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex;
- and
- (iii) determining whether a probe:target duplex is present;
- the presence of the duplex positively identifying the presence of the target organism in the test sample.
-23. (canceled)
Dependencies are inferred from the claim text.