Methods for forming tooth root and periodontal tissue unit, and regenerated tooth

A regenerated tooth comprising:

• a tooth root,
• wherein the regenerated tooth is obtained by a method which comprises forming a culture core containing the tooth and a cell-containing base material, the tooth being wrapped with the cell-containing base material, and culturing the culture core in a medium so as to form at least the tooth root in the tooth contained in the culture core,
• wherein the cell-containing base material contains at least one kind of cells selected from periodontal ligament-derived cells, bone marrow-derived cells, dental follicle-derived cells, dental pulp-derived cells and dental papilla-derived cells, and
• wherein the medium contains a component contained in a conditioned medium of a serum-free-cultured cell line of a human uterocervical squamous carcinoma cell line:
• an additive containing at least one selected from IL-1β, IL-6, IL-8, IL-9, EGF, IGF-I, GH, PDGF-AB, VEGF, LIF, HGF, FGF-2, FGF-1, BMP-2, BMP-4, M-CSF, dexamethasone, insulin, thyroxine, thyrocalcitonin, ascorbic acid and β-glycerophosphate;
• or both of the component and the additive.