Nucleotide sequences and corresponding polypeptides conferring modulated plant size and biomass and other characteristics in plants
AssigneeCeres, Inc.
InventorsKenneth Feldmann, Gregory Nadzan, Peter Mascia
Life of this patent
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Abstract
The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of modulated plant size, vegetative growth, organ number, plant architecture, sterility or seedling lethality in plants. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.
Claims22
An isolated nucleic acid molecule comprising:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of the polypeptides in FIGS. 1-73;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of the nucleotide sequences in the Sequence Listing;
- (d) a nucleotide sequence that is in reverse order of any one of the nucleotide sequences according to (c) when read in the 5′ to 3′ direction;
- (e) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (f) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (g) a nucleotide sequence encoding any one of the amino acid sequences corresponding to FIGS. 1-73.
- (h) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-73.
A method of modulating plant growth and phenotype characteristics, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of the polypeptides in FIGS. 1-73.
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of the nucleotide sequences in the Sequence Listing
- (d) a nucleotide sequence that is in reverse order of any one of the nucleotide sequences according to (c) when read in the 5′ to 3′ direction;
- (e) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (f) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (g) a nucleotide sequence encoding any one of the amino acid sequences in FIGS. 1-73;
- or
- (h) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-73, wherein said plant produced from said plant cell has modulated plant growth and phenotype characteristics as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A plant cell comprising an isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of the polypeptides in FIGS. 1-73.
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of the nucleotide sequences in the Sequence Listing;
- (d) a nucleotide sequence that is in reverse order of any one of the nucleotide sequences according to (c) when read in the 5′ to 3′ direction;
- (e) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (f) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (g) a nucleotide sequence encoding any one of the amino acid sequences in FIGS. 1-73, or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-73.
A method for promoting increased biomass in a plant, comprising:
- (a) transforming a plant with a nucleic acid molecule comprising a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in any one of FIGS. 1-73;
- and
- (b) expressing said nucleotide sequence in said transformed plant,
- whereby said transformed plant has an increased biomass as compared to a plant that has not been transformed with said nucleotide sequence.
Dependencies are inferred from the claim text.