Novel Methods of Diagnosis and Treatment of P. Aeruginosa Infection and Reagents Therefor
OwnerAndrew John Sloane, Susanne Kartin Pedersen +1inventors
InventorsAndrew John Sloane, Susanne Kartin Pedersen, Ron Weinberger
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel diagnostic, prognostic and therapeutic reagents for infection of an animal subject such as a human by Pseudomonas aeruginosa, and conditions associated with such infections, such as, for example, an acute clinical exacerbation in a cystic fibrosis (CF) subject. In particular, the present invention relates to methods for diagnosing/prognosing an infection by P. aeruginosa in a subject comprising detecting the presence or amount of one or more proteins of P. aeruginosa or a fragment or epitope thereof or an antibody thereto in a sample from the subject.
Claims61
A method for diagnosing an infection by Pseudomonas aeruginosa in a subject comprising detecting in a biological sample from said subject an immunogenic protein of P. aeruginosa or modified forma immunogenic fragment or epitope thereof, wherein the presence of said immunogenic protein, modified form, immunogenic fragment or epitope in the sample is indicative of infection.
(canceled)
(canceled)
A method for diagnosing an infection by Pseudomonas aeruginosa in a subject comprising detecting in a biological sample from said subject an antibody that binds to a protein of P. aeruginosa or a modified form, immunogenic fragment or epitope thereof, wherein said protein, modified form, immunogenic fragment or epitope generates an antibody response in the subject and wherein the presence of said antibody in the sample is indicative of infection and/or exacerbation.
(canceled)
(canceled)
32-62. (canceled)
A method of preparing a reagent for diagnosing an infection by P. aeruginosa and/or an acute clinical exacerbation, said method comprising obtaining one or more antibodies against a protein selected from the group consisting of ferric iron-binding protein (HitA), thioredoxin dependent reductase (PAPS), thioredoxin, heat shock protein GroES, nucleotide dependent kinase (NDK) and DNA-binding protein HU.
A method of preparing a reagent for diagnosing an infection by P. aeruginosa and/or an acute clinical exacerbation, said method comprising obtaining one or more proteins selected from the group consisting of ferric iron-binding protein (HitA), thioredoxin dependent reductase (PAPS), thioredoxin, heat shock protein GroES, nucleotide dependent kinase (NDK) and DNA-binding protein HU.
A method for eliciting the production of an antibody against P. aeruginosa in a subject comprising administering an isolated immunogenic protein of P. aeruginosa or an immunogenic fragment or epitope thereof to said subject for a time and under conditions sufficient to elicit the production of antibodies against the immunogenic protein, fragment or epitope.
-88. (canceled)
A vaccine comprising an immunogenic protein of P. aeruginosa or an immunogenic fragment or epitope thereof in combination with a pharmaceutically acceptable diluent.
A kit for detecting P. aeruginosa infection in a biological sample, said kit comprising:
- (i) one or more isolated antibodies that bind to an immunogenic protein of P. aeruginosa selected from the group consisting of ferric iron-binding protein (HitA), thioredoxin dependent reductase (PAPS), thioredoxin, heat shock protein GroES, nucleotide dependent kinase (NDK), DNA-binding protein HU or an immunogenic fragment or epitope thereof;
- and
- (ii) means for detecting the formation of an antigen-antibody complex.
A kit for detecting P. aeruginosa infection in a biological sample, said kit comprising:
- (i) one or more isolated immunogenic proteins of P. aeruginosa selected from the group consisting of ferric iron-binding protein (HitA), thioredoxin dependent reductase (PAPS), thioredoxin, heat shock protein GroES, nucleotide dependent kinase (NDK), DNA-binding protein HU or an immunogenic fragment or epitope thereof;
- and
- (ii) means for detecting the formation of an antigen-antibody complex.
Dependencies are inferred from the claim text.