Live, attenuated respiratory syncytial virus
AssigneeMerck Sharp & Dohme Corp.
InventorsMary K. Yagodich, Michael P. Citron, Daniel J. Distefano, Daniel L. Krah, Xiaoping Liang
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Abstract
The present invention features live, attenuated respiratory syncytial viruses (RSV) useful as vaccines against RSV infection and/or the development of severe RSV-associated illnesses. The disclosed viruses are attenuated to the extent of being nonpathogenic when administered to a subject but substantially retain the antigenic and immunogenic properties of wild-type RSV.
Claims32
A live, attenuated respiratory syncytial virus (RSV) comprising a viral genome, wherein the viral genome encodes proteins that comprise one or more amino acids selected from the group consisting of:
- a glutamic acid at position 204 of the protein encoded by the G gene;
- a glutamic acid at position 205 of the protein encoded by the G gene;
- an alanine at position 211 of the protein encoded by the G gene;
- a glutamic acid at position 213 of the protein encoded by the G gene;
- a glycine at position 221 of the protein encoded by the G gene;
- a glutamic acid at position 223 of the protein encoded by the G gene;
- a glycine at position 232 of the protein encoded by the G gene;
- a lysine at position 294 of the protein encoded by the F gene;
- a glycine at position 486 of the protein encoded by the F gene;
- an alanine at position 148 of the protein encoded by the L gene;
- and, a phenylalanine at position 2054 of the protein encoded by the L gene.
A live, attenuated respiratory syncytial virus (RSV) comprising a viral genome that comprises one or more nucleotide mutations compared to a non-attenuated RSV viral genome, wherein one or more of the nucleotide mutations result in one or more amino acid mutations, and wherein the one or more amino acid mutations are located at amino acid positions selected from group consisting of positions 204, 205, 211, 213, 221, 223, and 232, each of the protein encoded by the G gene;
- positions 294 and 486, each of the protein encoded by the F gene;
- and, positions 148 and 2054, each of the protein encoded by the L gene.
32-33. (canceled)
Dependencies are inferred from the claim text.