Recombinant chimeric antigens for diagnosis and prevention of scrub typhus
AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsWei-Mei Ching, Chien -Chung Chao
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Abstract
Recombinant chimeric antigens comprising unmodified and modified reactive polypeptide fragments of expressed product of the recombinant 56 kDa proteins of multiple strain of scrub typhus, such as Karp, Kato (Ktr56), Gilliam (Gmr56), and TA763 (TAr56). The invention is useful for detecting prior exposure to a number of strains of scrub typhus, based on the strength of reaction toward the chimeric protein and as a component in vaccine formulations and production of immune globulins for passive prophylaxis and immunity in subjects against heterologous infections.
Claims12
A recombinant chimeric protein comprising modified and unmodified reactive polypeptide fragments from more than one strains of Orientia tsutsugamushi.
An assay for detecting antibody to scrub typhus comprising:
- a. obtaining a sample from a subject;
- and
- b. exposing the sample to recombinant chimeric antigen in assay equipment selected from the group consisting of Elisa plates, dot-blot matrices, and hand held chromatographic and flow through assay devices;
- wherein said chimeric antigen containing unmodified and modified reactive polypeptide fragments from more than one strains of Orientia tsutsugamushi.
A method for inducing an immune response to Orientia tsutsugamushi comprising administering the chimeric antigen 1 is set forth in SEQ ID NO.:
- 1, SEQ ID NO.:
- 2 or SEQ ID NO.:
- 3. with a suitable pharmaceutical carrier to a subject.
Dependencies are inferred from the claim text.