Mesoderm and Definitive Endoderm Cell Populations
AssigneeMount Sinai School of Medicine of New York University
InventorsGordon M. Keller, Paul Gadue
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Abstract
The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy. The present invention further provides a method of generating hepatocytes, cell populations enriched for hepatocytes, and a method of hepatocyte replacement therapy.
Claims29
A method of making a cell population enriched for endoderm cells comprising culturing embryonic stem cells in the presence of activin or nodal and Wnt for about one to about six days, isolating brach+/HNF3β+ cells, and culturing said isolated cells in the presence of activin and an inhibitor of Wnt signalling for at least about one day.
A method of making a cell population enriched for endoderm cells comprising culturing human embryonic stem cells for about two to about ten days, isolating brach+/HNF3β+ cells, and culturing said brach+/HNF3β+ cells in the presence of activin or nodal and an inhibitor of Wnt signalling for at least about one day.
An embryonic stem cell in which a nucleic acid encoding a selectable marker is present in the HNF3β locus.
A method of making a cell population enriched for hepatocytes comprising culturing embryonic stem cells in the presence of activin or nodal to provide brach+/HFN3β3+/cKit+ cells, isolating the brach+/HFN3β3+/cKit+ cells and culturing said cells in the presence of BMP-4 and bFGF under conditions sufficient for the production of hepatoblasts, and culturing the hepatoblasts under conditions sufficient for the maturation of hepatoblasts to hepatocytes.
Dependencies are inferred from the claim text.