Fluorescence Detector for Microfluidic Diagnostic System
AssigneeHandyLab, Inc.
InventorsKalyan Handique, Sundaresh N. Brahmasandra
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Abstract
The present technology provides for an fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
Claims16
A diagnostic apparatus, comprising:
- one or more microfluidic channels configured to amplify one or more polynucleotides;
- and
- one or more fluorescence detectors configured to detect presence of the one or more polynucleotides in the one or more channels by detecting fluorescent light emitted from a probe associated with the one or more polynucleotides, wherein the one or more detectors each comprise:
- a first LED emitting light of a first color;
- a second LED emitting light of a second color;
- a first photodiode configured to collect emitted light of the first color;
- a second photodiode configured to collect emitted light of the second color;
- and wherein the first and second photodiodes are each connected to a pre-amplifier circuit having a time-constant of less than of about 1 second.
A diagnostic apparatus, comprising:
- one or more microfluidic channels configured to amplify one or more polynucleotides;
- and
- one or more fluorescence detectors configured to detect presence of the one or more polynucleotides in the one or more channels by detecting fluorescent light emitted from a probe associated with the one or more polynucleotides, wherein the one or more detectors each comprise:
- a LED emitting light of a specified color that excites the probe;
- a photodiode configured to collect emitted light of the specified color;
- and wherein the photodiode is connected to a pre-amplifier circuit having a time-constant of less than about 1 s.
A diagnostic apparatus, comprising:
- one or more microfluidic channels configured to amplify one or more polynucleotides;
- and
- one or more fluorescence detectors configured to detect presence of the one or more polynucleotides in the one or more channels by detecting fluorescent light emitted from a probe associated with the one or more polynucleotides, wherein the one or more detectors each comprise:
- a first LED emitting light of a first color;
- a second LED emitting light of a second color;
- a first photodiode configured to collect emitted light of the first color;
- a second photodiode configured to collect emitted light of the second color;
- and wherein the first and second photodiodes are each connected to a pre-amplifier circuit having a Gain of about 109.
A fluorescent detector, comprising:
- a LED emitting light of a specified color that excites a probe associated with one or more polynucleotides contained within a microfluidic channel;
- and
- a photodiode configured to collect emitted light of the specified color,
- wherein the photodiode is connected to a pre-amplifier circuit having a time-constant of less than about 1 s.
Dependencies are inferred from the claim text.