Novel sorting technology that allows for highly efficient selection of sperm without chromatin damage
AssigneeCornell University
InventorDarius A. Paduch
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Abstract
This invention provides a method to select and enrich healthy sperm for use in assisted reproductive techniques comprising incubating sperm with at least one marking reagent that enters unhealthy sperm but not healthy sperm. The invention also provides a method to sort healthy sperm of high viability from apoptotic, necrotic and dead sperm by using YO-PRO-1 and PI for fluorescence activated cell sorting in flow cytometers. A kit comprising YO-PRO-1 and PI is also included in this invention.
Claims11
A method to select healthy sperm for use in assisted reproductive technologies comprising:
- a) incubating sperm with at least one marking reagent that enters unhealthy sperm cells but not healthy sperm cells, and b) separating marked sperm from unmarked sperm.
A method of reducing unhealthy sperms in a semen sample for use in assisted reproductive technologies comprising:
- a) incubating said semen sample with at least one marking reagent that enters unhealthy sperm cells but not healthy sperm cells in said sample, and
- b) separating marked sperms from unmarked sperm, thereby reducing unhealthy sperms in said sample.
A kit comprising YO-PRO-1 and propidium iodide
Dependencies are inferred from the claim text.