Production of hemagglutinin-neuraminidase protein in microalgae
AssigneesMerial Limited, Sanofi Vaccine Technologies, S.A.S.
InventorsAnne-Cécile V. Bayne, James Casey Lippmeier, Kirk Emil APT, Xuan Guo, Joyce A. Pritchard
Life of this patent
Estimating the term…
Abstract
The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
Claims14
A method for production of a hemagglutinin-neuraminidase (HN) polypeptide, comprising culturing a recombinant microalgal host cell in a medium, wherein the recombinant microalgal host cell comprises a nucleic acid molecule comprising a polynucleotide sequence that encodes a heterologous HN polypeptide, to produce the heterologous HN polypeptide.
A method of producing a composition comprising a heterologous HN polypeptide, the method comprising:
- (a) expressing a heterologous HN polypeptide in a microalgal host cell, and
- (b) culturing the microalgal host cell under conditions sufficient to produce the heterologous HN polypeptide,
- wherein the composition is produced as the culture supernatant comprising the heterologous HN polypeptide.
A recombinant microalgal cell, comprising a nucleic acid molecule comprising a polynucleotide sequence that encodes a heterologous HN protein.
Dependencies are inferred from the claim text.