Transgenic sweet sorghum with altered lignin composition and process of preparation thereof
AssigneeNagarjuna Energy Private Limited
InventorsAsitava Basu, Mrinal K. Maiti, Satarupa Kar, Soumitra K. Sen, Banibrata Pandey
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Abstract
The present invention provides a sweet sorghum plant characterized by altered lignin content and/or altered lignin composition compared to a wild plant and this is achieved by manipulating the expression of caffeoyl-CoA-O-methyltransferase (CCoAOMT) in sweet sorghum by incorporation of a gene silencing construct comprising an isolated DNA sequence represented by SEQ ID NO 1.
Claims7
An isolated polynucleotide sequence obtained from Sorghum bicolor encoding Caffeoyl-CoA-O-methyltransferase (CCoAOMT) represented by SEQ ID NO 1.
A double strand mediated gene silencing cassette comprising a nucleic acid represented in SEQ ID NO 1, said nucleic acid is operably associated with a promoter, wherein the nucleic acid is in the sense-antisense orientation and facilitate to generate RNAi in vivo to at least a portion of a SEQ ID NO 1.
Forward and reverse primers represented by SEQUENCE ID NO 5 and 6.
Dependencies are inferred from the claim text.