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AssigneeSanofi-Aventis
InventorsBruno Dumas, Gilles Cauet, Tilman Achstetter, Eric Degryse
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The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20αHSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.
A method for modifying at least one steroid compound, comprising bringing the compound of a precursor thereof into contact with at least one Saccharomyces cerevisiae yeast strain comprising an inactivating genetic modification in both the YPR1 and GCY1 genes and comprising a reduced 20α-HSD type activity, or with a preparation derived from such a yeast.
A method for producing at least one steroidal derivative from at least one hydroxysteroid compound, comprising bringing at least one hydroxysteroid compound into contact with at least one Saccharomyces cerevisiae yeast strain comprising an inactivating genetic modification in both the YPR1 and GCY1 genes and comprising a reduced 20α-HSD type activity, or with a preparation derived from such a yeast.
9-12. (canceled)
A method for preparing at least one steroidal compound comprising the step of growing at least one Saccharomyces cerevisiae yeast strain comprising an inactivating genetic modification in both the YPR1 and GCY1 genes.
Dependencies are inferred from the claim text.