Endorphin Therapy Compositions and Methods
OwnerDipak Kumar Sarkarinventor
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Abstract
In certain embodiments, the invention provides methods of isolating and culturing neuronal stem cells from hypothalmi, methods of differentiating the neuronal cells into beta-endorphin neurons, and methods of treatment of various diseases comprising administering agents to differentiate endogenous neuronal stem cells into beta endorphin neurons.
Claims27
A method of isolating neural stem cells from a fetal hypothalamus comprising:
- isolating mixed neural and neural stem cells from glial cells in a fetal hypothalamus, and growing the isolated mixed neural and neural stem cells for several generations in cultures so that only the neural stem cells remain in the cultures.
A method of differentiating beta-endorphin neuronal cells from neural stem cells that are under the influence of LIF comprising:
- (i) removing the influence of LIF from the neural stem cells, (ii) maintaining the neural stem cells in an environment favoring the survival of neurons, and then (iii) treating the neural stem cells with a differentiating agent selected from group consisting of (a) pituitary adenylate cyclase activating peptide (PACAP), (b) dibutyryl cyclic adenylate cyclase (dbcAMP) and (c) a combination thereof.
A method of differentiating endogenous neural stem cells into BEP cells in a patient in need thereof comprising:
- (i) administering an effective amount of an agent selected from the group consisting of (a) pituitary adenylate cyclase activating peptide (PACAP), (b) dibutyryl cyclic adenylate cyclase (dbcAMP) and (c) a combination thereof into the central nervous system.
Dependencies are inferred from the claim text.