Method of replicating viruses in suspension cultures of dog kidney cells
OwnerMonty R. Warthen, William Skottainventors
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Abstract
Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process.
Claims22
A method of replicating virus in a culture of sCLDK cells comprising:
- a) inoculating a suspension of sCLDK cells with a virus, thereby infecting the sCLDK cells;
- b) allowing said virus to reproduce in said infected sCLDK cells;
- and
- c) harvesting said virus from said suspension culture of sCLDK cells.
A process of adapting substrate-dependent CLDK cells for growth in suspension comprising
- a) inoculating a sample of substrate-dependent CLDK cells in a medium comprising one or more serum substitutes;
- b) growing said cells in suspension in said medium;
- c) serially passaging said CLDK cells in suspension in fresh batches of said medium;
- and
- d) weaning said CLDK cells in suspension off of said one or more serum substitutes by reducing the amount of said serum substitutes in said medium to zero.
A method of continuously propagating sCLDK cells in suspension comprising:
- a) inoculating sCLDK cells into a medium;
- b) growing said sCLDK cells in suspension for a period of from about 4 to 10 days;
- c) transferring a sample of cultured material from (b) into fresh cell-free medium;
- d) and repeating (b) and (c) for a period of continuous growth.
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A composition comprising cell culture medium and sCLDK cells.
Dependencies are inferred from the claim text.