Bollworm Insect Resistance Management in Transgenic Plants
OwnerCarmen Sara Hernandez, Adri Van Vliet +2inventors
InventorsCarmen Sara Hernandez, Adri Van Vliet, Jeroen Van Rie, Juan Ferre Manzanero
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Abstract
This invention relates to the use of a combination of different proteins insecticidal to Helicoverpa zeaor Helicoverpa armigerain an insect resistance management process, wherein such proteins are: a) a Cry2A protein such as Cry2Aa, Cry2Ab, or Cry2Ae and b) a Cry1A, Cry1F or VIP3A protein, particularly wherein such proteins binds saturably to the insect midgut membrane of Helicoverpa zeaor Helicoverpa armigera, as well as plants and seeds expressing such combination of proteins, which are used to delay or prevent the development of resistance in populations of such insect species.
Claims20
A method for preventing or delaying insect resistance development in populations of the insect species Helicoverpa zea or Helicoverpa armigera to transgenic plants expressing insecticidal proteins to control said insect pest, said method comprising expressing a Cry2Ae, Cry2Aa, or Cry2Ab protein insecticidal to Helicoverpa zea or Helicoverpa armigera in combination with a VIP3, Cry1F or Cry1A protein insecticidal to Helicoverpa zea or Helicoverpa armigera in said plants, wherein said insecticidal proteins bind saturable and specific to receptor binding sites in said insects.
A method to control Helicoverpa zea or Helicoverpa armigera in a region where populations of said insect have become resistant to plants comprising a VIP3, a Cry1F or a Cry1A protein, comprising the step of sowing or planting in said region, plants comprising a Cry2Aa, Cry2Ab or Cry2Ae protein insecticidal to Helicoverpa zea or Helicoverpa armigera, wherein said insecticidal proteins bind saturable and specific to receptor binding sites in said insects.
A method to control Helicoverpa zea or Helicoverpa armigera in a region where populations of said insect have become resistant to plants comprising a Cry2Aa, Cry2Ab or Cry2Ae protein, comprising the step of sowing or planting in said region, plants comprising a VIP3, a Cry1F or a Cry1A protein insecticidal to Helicoverpa zea or Helicoverpa armigera, wherein said insecticidal proteins bind saturable and specific to receptor binding sites in said insects.
A method for obtaining plants comprising at least two different insecticidal proteins, wherein said proteins bind saturably and specifically to, and do not share binding sites in, larvae of the species Helicoverpa zea or Helicoverpa armigera as determined in competition binding experiments using brush border membrane vesicles of said insect larvae, comprising the step of obtaining plants comprising a plant-expressible chimeric gene encoding a Cry2Aa, Cry2Ab or Cry2Ae protein insecticidal to Helicoverpa zea or Helicoverpa armigera and a plant-expressible chimeric gene encoding a VIP3, Cry1F or Cry1A protein insecticidal to Helicoverpa zea or Helicoverpa armigera.
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A method for obtaining regulatory approval for planting or commercialization of plants expressing proteins insecticidal to H. zea or H. armigera, comprising the step of referring to, submitting or relying on insect assay binding data showing that Cry2A proteins do not compete with binding sites for Cry1A, Cry1F or VIP3 proteins in such insects.
A method for obtaining a reduction in structured refuge area containing plants not producing any protein insecticidal to H. zea or H. armigera in a field, such method comprising the step of referring to, submitting or relying on insect assay binding data showing that Cry2A proteins do not compete with binding sites for Cry1A, Cry1F or VIP3 proteins in such insects.
A field of insect-resistant transgenic plants controlling H. zea or H. armigera insects, wherein said field has a structured refuge area of less than 20%, of less than 15%, of less than 10%, or of less than 5%, or has no structured refuge area, wherein said plants express a combination of a Cry2Ae or Cry2Ab protein insecticidal to Helicoverpa armigera or Helicoverpa zea insects, and a Cry1A, Cry1F or VIP3A protein insecticidal to Helicoverpa armigera or Helicoverpa zea insects, particularly a Cry2Ae and a Cry1Ab, Cry1Ac or VIP3A protein insecticidal to Helicoverpa armigera or Helicoverpa zea insects, preferably a Cry2Ae, a Cry1Ab and a VIP3 protein, insecticidal to Helicoverpa armigera or Helicoverpa zea insects.
Dependencies are inferred from the claim text.