Cell signaling genes and related methods
AssigneeArborgen, LLC
InventorsShujun Chang, Marie B. Connett, Sarah Jane Emerson, Richard L. Forster, Katrina Gause, Ilkka Havukkala, Colleen Higgins, Robert Kodrzycki
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Abstract
Novel plant cell signaling genes and gene products are provided. These gene, polypeptide and oligonucleotide sequences are useful in regulating plant phenotype. Moreover, these genes and gene products are useful for expression profiling of plant cell signaling genes. The invention specifically provides polynucleotide and polypeptide sequences isolated from Eucalyptus and Pinus species.
Claims106
An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 and conservative variants thereof.
A DNA construct comprising at least one polynucleotide having the sequence of any one of SEQ ID NOs:
- 1-197 and conservative variants thereof.
An isolated polynucleotide comprising a nucleic acid sequence goo encoding the catalytic or substrate-binding domain of a polypeptide selected from of any one of SEQ ID NOs:
- 198-394, wherein the polynucleotide encodes a polypeptide having the activity of said polypeptide selected from any one of SEQ ID NOs:
- 198-394.
A method of making a transformed plant comprising:
- transforming a plant cell with a DNA construct comprising at least one polynucleotide encoding the catalytic or substrate-binding domain of a polypeptide selected from of any one of SEQ ID NOs:
- 198-394;
- and
- culturing the transformed plant cell under conditions that promote growth of a plant.
A method of making wood, comprising:
- transforming a plant with a DNA construct comprising a polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 and conservative variants thereof;
- culturing the transformed plant under conditions that promote growth of a plant;
- and
- obtaining wood from the plant.
A method of making wood pulp, comprising:
- transforming a plant with a DNA construct comprising a polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 and conservative variants thereof;
- culturing the transformed plant under conditions that promote growth of a plant;
- and
- obtaining wood pulp from the plant.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 198-394.
A method of altering a plant phenotype of a plant, comprising altering expression in the plant of a polypeptide encoded by any one of SEQ ID NOs:
- 1-197.
A polynucleotide comprising a nucleic acid selected from the group comprising of SEQ ID NOs:
- 395-583.
A method of correlating gene expression in two different samples, comprising:
- detecting a level of expression of one or more genes encoding a product encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 and conservative variants thereof in a first sample;
- detecting a level of expression of the one or more genes in a second sample;
- comparing the level of expression of the one or more genes in the first sample to the level of expression of the one or more genes in the second sample;
- and
- correlating a difference in expression level of the one or more genes between the first and second samples.
The method of 45, wherein the first sample and the second sample are obtained from plants in different stages of development.
A method of correlating the possession of a plant phenotype to the level of gene expression in the plant of one or more genes comprising:
- detecting a level of expression of one or more genes encoding a product encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 and conservative variants thereof in a first plant possessing a phenotype;
- detecting a level of expression of the one or more genes in a second plant lacking the phenotype;
- comparing the level of expression of the one or more genes in the first plant to the level of expression of the one or more genes in the second plant;
- and
- correlating a difference in expression level of the one or more genes between the first and second plants to possession of the phenotype.
A method of correlating gene expression to a response to an external stimulus or environmental condition, comprising:
- detecting a level of expression of one or more genes encoding a product encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 and conservative variants thereof in a first plant cell in the absence of the external stimulus or environmental condition;
- detecting a level of expression of the one or more genes in a second plant cell in the presence of the external stimulus or environmental condition;
- comparing the level of the expression of the one or more genes in the first plant cells to the level of expression of the one or more genes in the second plants cells;
- and
- correlating a difference in expression level of the one or more genes between the first and second samples to presence of the external stimulus or environmental condition.
A combination for detecting expression of one or more genes, comprising two or more oligonucleotides, wherein each oligonucleotide is capable of hybridizing to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197.
A combination for detecting expression of one or more genes, comprising two or more oligonucleotides, wherein each oligonucleotide is capable of hybridizing to a nucleic acid sequence encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197.
A method for detecting one or more genes in a sample comprising:
- contacting the sample with two or more oligonucleotides, wherein each oligonucleotide is capable of hybridizing to a gene comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 under standard hybridization conditions;
- and
- detecting the one or more genes of interest which are hybridized to the one or more oligonucleotides.
A method for detecting one or more nucleic acid sequences encoded by one or more genes in a sample, comprising:
- contacting the sample with two or more oligonucleotides, wherein each oligonucleotide is capable of hybridizing to a nucleic acid sequence encoded by a gene comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-197 under standard hybridization conditions;
- and
- detecting the one or more nucleic acid sequences which are hybridized to the one or more oligonucleotides.
Dependencies are inferred from the claim text.