Genes for improving salt tolerance and drought tolerance of plant and the uses thereof
AssigneeBiotechnology Research Institute, Chinese Academy of Agricultural Sciences
InventorsMin Lin, Ming Chen, Jin Wang, Jie Pan, Zhengfu Zhou, Wei Zhang, Wei Lu, Shuzhen Ping
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Abstract
Provide a nucleotide coding sequence and another nucleotide coding sequence artificially synthesized according to biased codons of plant. Construct recombinant vectors containing the genes as above and transform them into host cells including prokaryotic cells and eukaryotic cells. It is confirmed that the resulting transgenic plant has improved salt and drought tolerance after the said genes are expressed in the plant.
Claims8
DNA sequence for improving salt and drought tolerance of plant, which is set forth in SEQ ID NO:1.
DNA sequence for improving salt and drought tolerance of plant, which is set forth in SEQ ID NO:2.
A recombinant vector comprising DNA of SEQ ID NO:1 or SEQ ID NO:2.
A method for detecting whether there is DNA sequence of SEQ ID NO:1 or SEQ ID NO:2 in the samples, wherein the samples are hybridized with the antibody prepared with SEQ ID NO:1 to detect whether the antibody is reacted with the probe;
- the said samples are the product of PCR amplification, wherein the primers of PCR amplification correspond to SEQ ID NO:1 or SEQ ID NO:2 nucleotide encoding sequence and may be located in flank and middle of the coding sequence, the length of primer is 15-50 nucleotides.
Dependencies are inferred from the claim text.