Loading patent…
AssigneeFred Hutchinson Cancer Research Center
InventorsJames M. Roberts, Steven R. Coats, Matthew L. Fero
Estimating the term…
Hypercellular nonhuman organisms have functionally inactivated expression of a cyclin inhibitor gene, especially p27. The growth rate of nonhuman organisms are increased such that a desired size is attained more quickly than as compared to nonvariant organisms. Inhibitors of the p27 cyclin dependent kinase inhibitor protein or sequences encoding the protein modulate vertebrate cell cycle progression and increase the proportion of dividing cells to non-dividing cells in a population of treated cells. As the proportion of dividing cells increases, the cell population, e.g., hematopoietic progenitor (stem) cells, is more efficiently used for gene therapy applications. Transgenic animals and plants, and knockout alleles are provided.
A method for producing a hypertrophic variant organism, comprising functionally inactivating expression of a cyclin inhibitor gene in an organism wherein a hypertrophic variant is produced, the hypertrophy being relative to an organism having the functional cyclin inhibitor gene.
A polynucleotide targeting construct comprising a sequence that is homologous to a sequence present in a cyclin inhibitor gene and which, when integrated at the corresponding cyclin inhibitor gene locus, functionally inactivates cyclin inhibitor protein expression.
A hypertrophic nonhuman organism having a functionally inactivated cyclin inhibitor gene, the hypertrophy being relative to an organism having the functional cyclin inhibitor gene.
A method for increasing the growth rate of an nonhuman organism, comprising functionally inactivating expression of a cyclin inhibitor gene in an organism wherein the growth rate of the organism is increased relative to an organism of the same species having the functional cyclin inhibitor gene.
A method for increasing the proportion of dividing cells in a vertebrate cell population comprising:
A method for increasing the efficiency of transducing a vertebrate cell population with a viral vector encoding a gene product of interest, comprising:
A p27 inhibitor which comprises an oligonucleotide that specifically binds to DNA encoding p27 or RNA transcribed therefrom and inhibits expression of p27 protein.
Dependencies are inferred from the claim text.