Allergic disease model animals
AssigneeKeio University
InventorsMasayuki Amagai, Akiharu Kubo, Keisuke Nagao
Life of this patent
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Abstract
The object of the present invention is to provide a mouse model for allergic diseases such as atopic dermatitis, and a dermatitis mouse model with impaired skin-barrier function. The present inventors found out that a mouse that has been caused to completely lose the function of expressing profilaggrin protein and filaggrin protein by entirely or partially disrupting the endogenous gene encoding filaggrin by a genetic mutation such as deletion or replacement, can be used as a mouse model for allergic diseases or atopic dermatitis wherein the skin-barrier function has been impaired.
Claims4
1-12. (canceled)
An allergic-disease mouse model wherein a region from a translation-initiation site contained in exon 2 through an in-frame ATG contained in exon 3 is replaced by a marker gene due to a homologous recombination with a targeting vector having the following characteristics (a) to (c):
- (a) being designed so that the region from the translation-initiation site contained in exon 2 through the in-frame ATG contained in exon 3 of an endogenous gene encoding filaggrin is replaced by the marker gene at the time of homologous recombination;
- (b) comprising on a 5′ side of the marker gene sequence of the above (a), a gene sequence of 7 kb or more which is homologous to a sequence comprising a 5′-untranslated region of the endogenous gene encoding filaggrin;
- and
- (c) comprising on a 3′ side of the marker gene sequence of the above (a), a gene sequence of 1.5 kb or less which is homologous to a sequence downstream of the in-frame ATG contained in exon 3 of the endogenous gene encoding filaggrin.
Dependencies are inferred from the claim text.