Surface-coated structures and methods
AssigneeArch Biophysics, Inc.
InventorsRANDALL T. IRVIN, ELISABETH M. DAVIS, DONGYANG LI
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Abstract
The present invention relates to a method for covalently attaching a compound to a stainless steel, tin, iron, or titanium substrate, by contacting exposed surface(s) of the substrate with a synthetic pilin peptide containing a disulfide loop derived from the C-terminal receptor binding protein of Type IV P. aeruginosa (T4P) pilin, to bind the pilin peptide to the exposed surface(s), and covalently attaching the compound to the pilin peptide. Also disclosed are a substrate formed by the method and a biosensor device that uses the uses. Also disclosed are methods for improving the corrosion resistance, adhesive force, hardness and electron work function of certain metals.
Claims26
A method of covalently attaching a compound to one or more exposed surfaces of a substrate formed of stainless steel, tin, iron, or titanium, comprising contacting exposed surface(s) of the substrate with a synthetic pilin peptide containing a disulfide loop derived from the C-terminal receptor binding protein of Type IV P. aeruginosa (T4P) pilin and containing 0-10 additional residues on either or both the N- or C-terminal side of the loop, thereby to bind the pilin peptide covalently to the exposed surface(s), and
- before of after said contacting and binding, covalently attaching the compound to the pilin peptide.
In a method of treating stainless steel, tin, iron, or titanium metal material having surfaces with exposed grain boundary regions, to reduce the rate of corrosion or the material, an improvement comprising
- contacting exposed surfaces boundary regions in the material with a synthetic pilin peptide containing a disulfide loop derived from the C-terminal receptor binding protein of Type IV P. aeruginosa (T4P) pilin and containing 0-10 additional residues on either or both the N- or C-terminal side of the loop, under conditions effective to change the electron work function of exposed grain-boundary regions by at least 0.2 EFW units and to increase the hardness of exposed grain-boundary regions, as measured by nano-indentation produced striking the metal surface with the tip of an atomic force microscope with a given force, by at least 20%.
A method of inhibiting an inflammatory response against a medical device that is designed to be implanted in a subject, comprising,
- prior to implanting the device, coating exposed surfaces of the device with a synthetic pilin peptide containing (i) a disulfide loop derived from the C-terminal receptor binding protein of Type IV P. aeruginosa (T4P) pilin, (ii) 0-10 additional residues on either or both the N- or C-terminal side of the loop, and (iii) composed of D-amino acids, mixture of D- and L-amino acids, or D-amino acids in a retro-inverso (RI) form.
A medical device having surfaces that are exposed to inflammatory-response cells when implanted in a body, in which such surfaces are coated with a synthetic pilin peptide containing (i) a disulfide loop derived from the C-terminal receptor binding protein of Type IV P. aeruginosa (T4P) pilin, (ii) 0-10 additional residues on either or both the N- or C-terminal side of the loop, and (iii) composed of D-amino acids, mixture of D- and L-amino acids, or D-amino acids in a retro-inverso (RI) form.
Dependencies are inferred from the claim text.