Nucleoside and nucleotide having an unnatural base and use thereof
AssigneeRiken
InventorsIchiro Hirano, Shigeyuki Yokoyama
Life of this patent
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Abstract
The object of the present invention is to provide a nucleoside or a nucleotide, or a derivative thereof, which has an unnatural base. The nucleoside and others of the present invention are characterized by having a 2-amino-6-(2-thiazolyl)purin-9-yl group or a 2-amino-6-(2-oxazolyl)purin-9-yl group as a base, wherein the 4- and/or 5-position of the thiazolyl or oxazolyl group may be substituted.
Claims13
A nucleoside or a nucleotide, or a derivative thereof, which has a 2-amino-6-(2-thiazolyl)purin-9-yl group or a 2-amino-6-(2-oxazolyl)purin-9-yl group as a base, wherein the 4- and/or 5-position of the thiazolyl or oxazolyl group may be substituted.
A method for preparing a nucleic acid incorporating a nucleotide having a 5-substituted or unsubstituted-2-oxo(1H)-pyridin-3-yl group as a base, which comprises:
- effecting transcription, replication or reverse transcription by using, as a template, a nucleic acid containing a nucleotide having a 2-amino-6-(2-thiazolyl)purin-9-yl group or a 2-amino-6-(2-oxazolyl)purin-9-yl group as a base, wherein the 4- and/or 5-position of the thiazolyl or oxazolyl group may be substituted, so that the nucleotide having a 5-substituted or unsubstituted-2-oxo(1H)-pyridin-3-yl group as a base is incorporated at a site complementary to the nucleotide having a 2-amino-6-(2-thiazolyl)purin-9-yl group or a 2-amino-6-(2-oxazolyl)purin-9-yl group as a base.
Dependencies are inferred from the claim text.