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US 2011/0087011 A1Published application
Separation of rebaudioside a from stevia glycosides using chromatography
AssigneeCargill, Incorporated
InventorsChen-Chou Chiang, Jeffrey C. Evans, John Joseph Hahn, An Amanda Jules Heylen, Andrew Keith Ohmes, Alexander Patist, Troy Allen Rhonemus, Jenna Stangler, Christopher Austin Tyler, Ronny Leontina Marcel Vercauteren
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Abstract
Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.
Claims23
A method of separating rebaudioside A from stevioside using chromatography, the method comprising the steps of:
- (a) providing a glycoside solution comprising rebaudioside A;
- stevioside;
- and a solvent;
- (b) providing an adsorbent comprising a polystyrene divinylbenzene support functionalized with tertiary amines, quaternary amines, or both tertiary and quaternary amines;
- and
- (c) contacting the adsorbent with the glycoside solution in order to separate at least a portion of the rebaudioside A from the stevioside thereby resulting in the formation of a purified rebaudioside A solution.
3 more elements
Dependencies are inferred from the claim text.