Methods for purification of alpha-1-antitrypsin andapolipoprotein a-1
AssigneeCSL Behring GmbH
InventorsNathan Brinkman, Douglas Bigler, Reinhard Bolli, Vreni Foertsch
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Abstract
This invention relates to protein separation and purification methods for both alpha-1-antitrypsin (AAT, also known as alpha-1 proteinase inhibitor, API, and A1-PI) and Apolipoprotein A-I (ApoA-1) from, for example, a fraction of human blood plasma. In certain embodiments, the invention provides methods for separating AAT from ApoA-1 at the initial stage of purification, so that the same starting material can be used as a source for both proteins. The methods further pertain to providing compositions of AAT and of ApoA-1 suitable for pharmaceutical use and are suitable for large-scale purification.
Claims25
A method for purifying Apolipoprotein A-I (ApoA-1) and Alpha-1-Antitrypsin (AAT) from a single starting human plasma fraction containing both proteins comprising:
- i) treating a starting human plasma fraction comprising ApoA-1 and AAT to separate an ApoA-1 containing fraction from an AAT containing fraction;
- ii) purifying ApoA-1 to pharmaceutical grade purity from the ApoA-1 containing fraction;
- and
- iii) purifying AAT to pharmaceutical grade purity from the AAT containing fraction.
Dependencies are inferred from the claim text.