Methods for Cyclizing Synthetic Polymers
AssigneeRigel Pharmaceuticals, Inc.
InventorTodd M. Kinsella
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Abstract
The invention provides methods and compositions for production of a cyclic polymer in a cell free system. In general, the methods of the invention involve ligating first and second recombinant intein domains to a linear synthetic polymer to form a compound containing the structure: D1-X(n)-D2, where D1 is a first catalytic domain of an intein; D2 is a second catalytic domain of an intein; where the second catalytic domain has at its N-terminus a first reactive site for the intein; and X(n) is a polymer of a number n of monomer X, where the polymer N-terminus has a second reactive site for the intein. D1-X(n)-D2 compounds autocatalytically cyclize the X(n) polymer to produce a cyclic polymer. The invention finds use in a variety of drug discovery, clinical and therapeutic applications.
Claims10
1-18. (canceled)
A method for making a cyclic polymer, comprising:
- incubating a compound of the structure:
- D1-X(n)-D2
- wherein D1 is a first catalytic domain of an intein;
- wherein X(n) is polymer comprising n residues of a monomer X, n is at least 2, the polymer is not genetically encodable, and the polymer has a first reactive site for said intein at its N-terminus;
- and
- wherein D2 is a second catalytic domain of an intein, having at its N-terminus a second reactive site for said intein;
- under intein reaction conditions to produce said cyclic polymer.
Dependencies are inferred from the claim text.