High eicosapentaenoic acid producing strains of yarrowia lipolytica

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one gene encoding Δ15 desaturase;
• b) at least one gene encoding Δ6 desaturase;
• c) at least one gene encoding C18/20 elongase;
• and,
• d) at least one gene encoding Δ5 desaturase.

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one gene encoding Δ9 elongase;
• b) at least one gene encoding Δ8 desaturase;
• c) at least one gene encoding Δ5 desaturase;
• and,
• d) at least one gene encoding Δ17 desaturase.

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one gene encoding Δ5 desaturase;
• b) at least one gene encoding Δ9 elongase;
• c) at least one gene encoding Δ8 desaturase;
• and,
• d) at least one gene encoding Δ5 desaturase.

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one gene encoding Δ6 desaturase;
• b) at least one gene encoding C18/20 elongase;
• c) at least one gene encoding Δ5 desaturase;
• d) at least one gene encoding Δ17 desaturase;
• e) at least one gene encoding C16/18 elongase;
• and,
• f) at least one gene encoding Δ12 desaturase;
• and
• wherein the background Yarrowia sp. is devoid of any native gene encoding an enzyme selected from the group consisting of:
• peroxisomal acyl-CoA oxidase ACO3 (Pox3-), lipase 1 (Lip1-) and Δ12 desaurase.

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one gene encoding Δ9 elongase;
• b) at least one gene encoding Δ8 desaturase;
• c) at least one gene encoding Δ5 desaturase;
• d) at least one gene encoding Δ17 desaturase;
• e) at least one gene encoding C16/18 elongase;
• and,
• f) at least one gene encoding Δ12 desaturase;
• and
• wherein the background Yarrowia sp. is devoid of any native gene encoding an enzyme selected from the group consisting of:
• acyl-CoA oxidase 2 (Pox2-), and saccharopine dehydrogenase (Lys5-).

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one gene encoding Δ15 desaturase;
• b) at least one gene encoding Δ9 elongase;
• c) at least one gene encoding Δ8 desaturase;
• d) at least one gene encoding Δ5 desaturase;
• and,
• e) at least one gene encoding Δ12 desaturase.

A recombinant production host cell for the production of eicosapentaenoic acid comprising a background Yarrowia sp. comprising a gene pool comprising the following genes of the ω-3/ω-6 fatty acid biosynthetic pathway:

• a) at least one set of genes selected from the group consisting of:
• (1) at least one gene encoding Δ6 desaturase;
• at least one gene encoding C18/20 elongase;
• at least one gene encoding Δ5 desaturase;
• and, at least one gene encoding Δ17 desaturase;
• (2) at least one gene encoding Δ9 elongase;
• at least one gene encoding Δ8 desaturase;
• at least one gene encoding Δ5 desaturase;
• and, at least one gene encoding Δ17 desaturase;
• (3) at least one gene encoding Δ15 desaturase;
• at least one gene encoding Δ6 desaturase;
• at least one gene encoding C18/20 elongase;
• and, at least one gene encoding Δ5 desaturase;
• (4) at least one gene encoding Δ15 desaturase;
• at least one gene encoding Δ9 elongase;
• at least one gene encoding Δ8 desaturase;
• at least one gene encoding Δ5 desaturase;
• and,
• b) at least one gene encoding an enzyme selected from the group consisting of:
• (1) Δ12 desaturase;
• (2) Δ9 desaturase;
• (3) C14/16 elongase;
• (4) C16/18 elongase;
• and,
• c) at least one gene encoding an enzyme selected from the group consisting of:
• (i) diacylglycerol acyltransferase selected from the group consisting of DGAT1, DGAT2 and PDAT;
• (ii) acyl-CoA:1-acyl lysophosphatidylcholine acyltransferase (LPCAT);
• (iii) glycerol-3-phosphate acyltransferase (GPAT);
• (iv) lysophosphatidic acid acyltransferase (LPAAT);
• (v) phospholipase C;
• and,
• (vi) phospholipase A2;
• wherein:
• (1) the background Yarrowia sp. is devoid of any native gene encoding a polypeptide having Δ12 desaturase activity;
• and,
• (2) the background Yarrowia sp. is devoid of any native gene encoding an enzyme selected from the group consisting of lipase 1 (Lip1-), peroxisomal acyl CoA oxidase ACO3 (Pox3-), acyl-CoA oxidase 2 (Pox2-), orotidine-5′-phosphate decarboxylase (Ura3-), saccharopine dehydrogenase (Lys5-), lipase 2 (Lip2-) and isopropyl malate dehydrogenase (Leu2-).