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AssigneeSignature Genomics Laboratories, LLC
InventorsLisa McDaniel, Blake Ballif, Roger Schultz, Brice Tebbs, Bassem Bejjani, Lisa Shaffer
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Multiplex (+/−) stranded analyses, such as array comparative genomic hybridization (aCGH), are provided for detecting chromosomal rearrangements associated with cancer and other diseases. For example, an illustrative multiplex array for CGH includes discrete plus (+) strand and minus (−) strand DNA probes, complementary to each other but separable on the CGH array. The minus (−) strand DNA probes recover diagnostic information lost to conventional microarrays, since many genes transcribe from the minus (−) strand. In an illustrative system, patient and control DNA samples are prepared for CGH by amplification and labeling using comprehensive primers that generate both plus (+) strands and minus (−) strands of DNA in the samples. The breakpoints of a translocated chromosome may be detected on a multiplex microarray by DNA probes of one polarity, while DNA copy number changes associated with the translocation region may be detected by corresponding DNA probes of the complementary polarity. Related methods for identifying translocation partner genes are also provided.
A method for detecting chromosomal rearrangements, comprising receiving a DNA sample and analyzing the DNA sample via comparative genomic hybridization for chromosomal rearrangements using an array of plus (+)-stranded DNA probes and minus (−)-stranded DNA probes.
A method for detecting chromosomal rearrangements, comprising:
A method for detecting chromosomal rearrangements, comprising:
An array for the detection of chromosomal abnormalities comprising plus (+)-stranded DNA probes and minus (−)-stranded DNA probes.
Dependencies are inferred from the claim text.