Biochip
AssigneeAutogenomics, Inc.
InventorsVijay Mahant, Fareed Kureshy
Life of this patent
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Abstract
Improved biochips comprise a matrix layer coupled to a substrate, wherein the matrix layer includes a plurality of ligands in a plurality of predetermined positions and wherein ligands bind to an anti-ligand disposed in a sample fluid. Preferred matrix layers are multi-functional matrix layers that reduce autofluorescence, incident-light-absorption, charge-effects, and/or surface unevenness of the substrate, and contemplated biochips may comprise additional matrix layers. Contemplated biochips may be useful in detection and/or quantification of various anti-ligands, including polypeptides, polynucleotides, carbohydrates, pharmacologically active molecules, bacterial or eukaryotic cells, and/or viruses.
Claims20
A method of forming a biochip, comprising:
- coating onto an organic polymer substrate a hydrophilic layer to form a hydrophilic surface on the substrate;
- applying onto the hydrophilic surface a solution comprising (1) a light-absorbing agent absorbs light at a wavelength of autofluorescence of the substrate and (2) a first dissolved organic polymer or a first plurality of organic monomers, and forming in a gelling or polymerization reaction a first matrix layer on the hydrophilic surface from the solution;
- applying onto the first matrix layer a solution comprising (1) a first portion of a coupling moiety that allows specific and non-covalent binding of a second portion of the coupling moiety and (2) a second dissolved organic polymer or a second plurality of organic monomers, and forming in a gelling or polymerization reaction a second matrix layer on the first matrix layer from the solution, wherein the first portion is distributed throughout the entire second matrix layer;
- applying onto the second matrix layer a solution comprising (1) a detergent and (2) a third dissolved organic polymer or a third plurality of organic monomers, and forming in a gelling or polymerization reaction a third matrix layer on the second matrix layer from the solution;
- applying onto the third matrix layer a plurality of distinct ligands in predetermined respective positions, wherein each of the plurality of distinct ligands comprises the second portion of the coupling moiety, and wherein each of the plurality of distinct ligands is retained at the site of application by the first portion.
Dependencies are inferred from the claim text.