Method for detecting antigen, and apparatus for detecting antigen using the same, and microfluidic chip using the same

Abstract

A method for detecting an antigen, an apparatus for detecting an antigen using the same, and a microfluidic chip using the same are disclosed. The method for detecting an antigen, includes: binding a first antibody and nano-beads to generate antibody nano-beads; binding the generated antibody nano-beads and an antigen to generate antigen-antibody nano-beads; forming at least one of an electric field and a magnetic field on the generated antigen-antibody nano-beads to bind the generated antigen-antibody nano-beads and a second antibody; and detecting the antigen-antibody nano-beads bound to the second antibody. Thus, when nano-beads affected by an electromagnetic field exist within the electromagnetic field that temporally and spatially changes, the nano-beads move according to non-uniformity of the electromagnetic field. In particular, an active mixing can be performed by using the electromagnetic field which is spatially non-uniform and changes temporally, a reaction time can be reduced. In addition, a flow can be controlled to make nano-beads move to a capture antibody, thus detecting an antigen with a small amount of a test sample within a short time.

Dependencies are inferred from the claim text.