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AssigneeCorning Incorporated
InventorsRonald A. Faris, Vasiliy N. Goral, Miya (Yi-Cheng) Hsieh, Odessa N. Petzold, Po Ki Yuen
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A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.
A cell culture apparatus comprising:
A cell culture apparatus according to any of the preceding claims, further comprising a second perfusion channel forming a plurality of openings in communication with the cell retention chamber, the openings being of a dimension configured to prevent cells from the culture chamber from entering the second perfusion channel.
A cell culture apparatus according to any of the preceding claims, wherein the cell retention chamber has a width of between 80 micrometers and 110 micrometers.
A cell culture apparatus according to any of the preceding claims, wherein the cell retention chamber has a height of between 40 micrometers and 50 micrometers.
A cell culture apparatus according to any of the preceding claims, wherein the first perfusion channel has a height of between 30 micrometers and 50 micrometers and has a width of between 30 micrometers to 50 micrometers.
A method for culturing cells in an apparatus according to any of the preceding claims, the method comprising:
A cell culture apparatus comprising:
Dependencies are inferred from the claim text.