Methods for producing enucleated erythroid cells derived from pluripotent stem cells
AssigneeAdvanced Cell Technology, Inc.
InventorsRobert Lanza, Shi-Jiang Lu
Life of this patent
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Abstract
Methods for generating enucleated erythroid cells using pluripotent stem cells are provided. The methods permit the production of large numbers of cells. The cells obtained by the methods disclosed may be used for a variety of research, clinical, and therapeutic applications. Methods for generating megakaryocyte and platelets are also provided.
Claims47
A method of producing a pluripotent stem cell-derived enucleated erythroid cell, comprising:
- providing a pluripotent stem cell;
- and
- differentiating said pluripotent stem cell into an enucleated erythroid cell by culturing said pluripotent stem cell with OP9 mouse stromal cells or human mesenchymal stem cells (MSCs).
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A method of producing a pluripotent stem cell-derived erythroid cell, comprising:
- providing a pluripotent stem cell;
- and
- differentiating said pluripotent stem cell into an erythroid cell by culturing said pluripotent stem cell in a medium comprising EPO.
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A method of producing a megakaryocyte or a platelet, comprising:
- providing a pluripotent stem cell;
- differentiating said pluripotent stem cell into a hemangioblast, non-engrafting hemangio cell, or blast cell;
- and
- differentiating said hemangioblast, non-engrafting hemangio cell, or blast cell into said megakaryocyte or said platelet by culturing in megakaryocyte (MK) culture medium comprising TPO.
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Dependencies are inferred from the claim text.